| Team | Riders | Team launch |
| Ducati | Jack Miller, Francesco Bagnaia | February 9 |
| Pramac Ducati | Johann Zarco, Jorge Martin | February 25 |
| Esponsorama/VR46 Ducati | Luca Marini, Enea Bastianini | February 5 |
| Yamaha | Maverick Vinales, Fabio Quartararo | February 15 |
| Petronas Yamaha SRT | Franco Morbidelli, Valentino Rossi | March 1 |
| Suzuki | Joan Mir, Alex Rins | March 6 |
| KTM | Brad Binder, Miguel Oliveira | February 12 |
| Tech3 KTM | Danilo Petrucci, Iker Lecuona | February 12 |
| Honda | Pol Espargaro, Marc Marquez | February 22 |
| LCR Honda | Takaaki Nakagami, Alex Marquez | February 19 (AM) February 20 (TN) |
| Aprilia | Aleix Espargaro, TBC | March 4 |
Following the cancellation of the planned first pre-season test at Sepang, MotoGP will hold its official pre-season testing in Qatar on March 6-7 and March 10-12. There will be a shakedown test for rookies, test riders and MotoGP’s sole remaining concession factory team Aprilia on March 5.
Latest 2021 calendar
28 March Qatar – Losail
4 April Qatar – Losail
18 April Portugal – Portimao
2 May – Spain – Jerez
16 May France – Le Mans
30 May Italy – Mugello
6 June Catalunya – Barcelona
20 June Germany – Sachsenring
27 June Netherlands – Assen
11 July Finland – KymiRing
15 August Austria – Red Bull Ring
29 August Great Britain – Silverstone
12 September Aragon
19 September San Marino – Misano
3 October Japan – Motegi
10 October Thailand – Chang International
24 October Australia – Phillip Island
31 October Malaysia – Sepang
14 November Valencia
Reserve: Indonesia (Mandalika)
Postponed indefinitely: USA (Austin), Argentina (Termas de Rio Hondo)
